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Figure 9. Effect of lactobacilli strains on the production of inflammatory cytokines and chemokines by murine Peyer’s patches macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticaseibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, Peyer’s patches macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by <t>ELISA.</t> Peyer’s patches of macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

Csf3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506."

Article Title: Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506.

Journal: International journal of molecular sciences

doi: 10.3390/ijms26062688

Figure Legend Snippet: Figure 9. Effect of lactobacilli strains on the production of inflammatory cytokines and chemokines by murine Peyer’s patches macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticaseibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, Peyer’s patches macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peyer’s patches of macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

Techniques Used: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

Figure 10. Effect of lactobacilli strains on the production of inflammatory and regulatory cytokines by murine Peyer’s patches macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticaseibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, Peyer’s patches macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peyer’s patches macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

Figure Legend Snippet: Figure 10. Effect of lactobacilli strains on the production of inflammatory and regulatory cytokines by murine Peyer’s patches macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticaseibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, Peyer’s patches macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peyer’s patches macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

Techniques Used: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

Figure 11. Effect of lactobacilli strains on the production of inflammatory cytokines and chemokines by murine peritoneal macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysac- charide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticas- eibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, peritoneal macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peritoneal macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

Figure Legend Snippet: Figure 11. Effect of lactobacilli strains on the production of inflammatory cytokines and chemokines by murine peritoneal macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysac- charide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticas- eibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, peritoneal macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peritoneal macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

Techniques Used: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

Figure 12. Effect of lactobacilli strains on the production of inflammatory and regulatory cytokines by murine peritoneal macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysac- charide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticas- eibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, peritoneal macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peritoneal macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

Figure Legend Snippet: Figure 12. Effect of lactobacilli strains on the production of inflammatory and regulatory cytokines by murine peritoneal macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysac- charide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticas- eibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, peritoneal macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peritoneal macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

Techniques Used: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

Enzyme-linked Immunosorbent Assay:

Article Title: Disruption of MerTK increases the efficacy of checkpoint inhibitor by enhancing ferroptosis and immune response in hepatocellular carcinoma
Article Snippet: .. Double-blinded quantitative detection of IFN-γ and CSF3 were performed using the mouse IFN-γ ELISA kit (R&D Systems, USA) and CSF3 ELISA kit (R&D Systems, USA) according to the manufacturer’s instructions. ..

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PPARα acts as an upstream positive regulator of FADS2 in psoriatic keratinocytes. A) RT‐qPCR analysis of PPARA expression in normal skin from healthy controls (NN, n = 4) and lesional skin tissue from psoriasis patients (PS, n = 5). B) Representative immunofluorescence images of PPARα and K14 co‐staining in normal skin from healthy controls and lesional skin tissue from psoriasis patients. C) RT‐qPCR analysis of PPARA in psoriatic lesional skin before and 10 weeks after infliximab treatment (n = 5). D) Representative images of PPARα immunofluorescence staining in lesional skin from psoriasis patients at baseline and week 10 following infliximab treatment. E) Representative immunofluorescence images of PPARα staining in healthy skin from control mice and skin lesions from IMQ‐induced psoriasis mouse model. F,G) RT‐qPCR analysis of PPARA (F) and FADS2 (G) expression in HaCaT cells stimulated with M5 for 12 h (n = 3). H) Immunoblotting of PPARα and FADS2 in HaCaT cells stimulated with M5 cytokines for the indicated time. I) RT‐qPCR analysis of PPARA , FADS2 , and indicated genes in HaCaT cells transfected with PPARA siRNA (si PPARA ) and control siRNA (siNC) for 24 h, followed by PBS or M5 stimulation for 12 h (n = 3). J) RT‐qPCR analysis of FADS2 and indicated genes in HaCaT cells pretreated with WY14643 or DMSO for 21 h, followed by PBS or M5 stimulation for 3 h (n = 3). K) ELISA quantification of CXCL1, CXCL8, and CSF3 protein levels in cell lysates and supernatants from HaCaT cells treated as in (J) (n = 3). L) RT‐qPCR analysis of the indicated genes in HaCaT cells transfected with si FADS2 and siNC for 24 h, followed by M5 stimulation for 3 h before WY14643 or DMSO pretreatment (n = 3). Scale bar, 50 µm. Data are presented as mean ± SD. Statistical significance was determined by unpaired two‐tailed Student's t test (A,F,G,K), paired two‐tailed Student's t test (C), or one‐way ANOVA (I,J,L). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, not significant.

Journal: Advanced Science

Article Title: Reprogramming of Fatty Acid Metabolism via PPARα‐Orchestrated FADS2 in Keratinocytes Modulates Skin Inflammation in Psoriasis

doi: 10.1002/advs.202417049

Figure Lengend Snippet: PPARα acts as an upstream positive regulator of FADS2 in psoriatic keratinocytes. A) RT‐qPCR analysis of PPARA expression in normal skin from healthy controls (NN, n = 4) and lesional skin tissue from psoriasis patients (PS, n = 5). B) Representative immunofluorescence images of PPARα and K14 co‐staining in normal skin from healthy controls and lesional skin tissue from psoriasis patients. C) RT‐qPCR analysis of PPARA in psoriatic lesional skin before and 10 weeks after infliximab treatment (n = 5). D) Representative images of PPARα immunofluorescence staining in lesional skin from psoriasis patients at baseline and week 10 following infliximab treatment. E) Representative immunofluorescence images of PPARα staining in healthy skin from control mice and skin lesions from IMQ‐induced psoriasis mouse model. F,G) RT‐qPCR analysis of PPARA (F) and FADS2 (G) expression in HaCaT cells stimulated with M5 for 12 h (n = 3). H) Immunoblotting of PPARα and FADS2 in HaCaT cells stimulated with M5 cytokines for the indicated time. I) RT‐qPCR analysis of PPARA , FADS2 , and indicated genes in HaCaT cells transfected with PPARA siRNA (si PPARA ) and control siRNA (siNC) for 24 h, followed by PBS or M5 stimulation for 12 h (n = 3). J) RT‐qPCR analysis of FADS2 and indicated genes in HaCaT cells pretreated with WY14643 or DMSO for 21 h, followed by PBS or M5 stimulation for 3 h (n = 3). K) ELISA quantification of CXCL1, CXCL8, and CSF3 protein levels in cell lysates and supernatants from HaCaT cells treated as in (J) (n = 3). L) RT‐qPCR analysis of the indicated genes in HaCaT cells transfected with si FADS2 and siNC for 24 h, followed by M5 stimulation for 3 h before WY14643 or DMSO pretreatment (n = 3). Scale bar, 50 µm. Data are presented as mean ± SD. Statistical significance was determined by unpaired two‐tailed Student's t test (A,F,G,K), paired two‐tailed Student's t test (C), or one‐way ANOVA (I,J,L). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, not significant.

Article Snippet: Similarly, human CXCL1 (#70‐EK196), CSF3 (#70‐EK169), and CXCL8 (#70‐EK108/2) levels in cultured human keratinocyte lysates and supernatants were measured using corresponding human ELISA kits (Lianke Bio, China) according to the provided protocols.

Techniques: Quantitative RT-PCR, Expressing, Immunofluorescence, Staining, Control, Western Blot, Transfection, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: International journal of molecular sciences

Article Title: Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506.

doi: 10.3390/ijms26062688

Figure Lengend Snippet: Figure 9. Effect of lactobacilli strains on the production of inflammatory cytokines and chemokines by murine Peyer’s patches macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticaseibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, Peyer’s patches macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peyer’s patches of macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

Article Snippet: 2025, 26, 2688 22 of 26 BMS614) and IL-12 (Mouse IL-12 p70 ELISA Kit, BMS6004) from ThermoFisher Scientific (Waltham, MA, USA), and CCL-2 (Mouse CCL2/JE/MCP-1 ELISA Kit Quantikine, MJE00B), CCL-8 (Mouse CCL8/MCP-2 DuoSet ELISA, DY790), IL-27 (Mouse IL-27 p28/IL-30 ELISA Kit Quantikine, M2728), CSF2 (Mouse GM-CSF DuoSet ELISA, DY415), and CSF3 (Mouse G-CSF ELISA Kit Quantikine, MCS00) from R&D Systems (Minneapolis, MN, USA).

Techniques: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: International journal of molecular sciences

Article Title: Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506.

doi: 10.3390/ijms26062688

Figure Lengend Snippet: Figure 10. Effect of lactobacilli strains on the production of inflammatory and regulatory cytokines by murine Peyer’s patches macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticaseibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, Peyer’s patches macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peyer’s patches macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

Techniques: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: International journal of molecular sciences

Article Title: Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506.

doi: 10.3390/ijms26062688

Figure Lengend Snippet: Figure 11. Effect of lactobacilli strains on the production of inflammatory cytokines and chemokines by murine peritoneal macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysac- charide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticas- eibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, peritoneal macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peritoneal macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

Techniques: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: International journal of molecular sciences

Article Title: Modulation of Macrophages TLR4-Mediated Transcriptional Response by Lacticaseibacillus rhamnosus CRL1505 and Lactiplantibacillus plantarum CRL1506.

doi: 10.3390/ijms26062688

Figure Lengend Snippet: Figure 12. Effect of lactobacilli strains on the production of inflammatory and regulatory cytokines by murine peritoneal macrophages stimulated with the Toll-like receptor 4 (TLR4) agonist lipopolysac- charide (LPS). Immunocompetent adult BALB/c mice (6 weeks) were orally treated with Lacticas- eibacillus rhamnosus CRL1505 or Lactiplantibacillus plantarum CRL1506 strains for five days. On day 6, peritoneal macrophages were isolated, cultured for 24 h, and then challenged with LPS. The levels of cytokines were determined after 24 h of culture (basal) or 24 h after LPS challenge by ELISA. Peritoneal macrophages obtained from non-lactobacilli-treated mice were used as controls. Asterisks indicate significant differences between the indicated groups and basal or LPS-challenged controls, (*) p < 0.05; (**) p < 0.01.

Techniques: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Cell Reports Medicine

Article Title: Disruption of MerTK increases the efficacy of checkpoint inhibitor by enhancing ferroptosis and immune response in hepatocellular carcinoma

doi: 10.1016/j.xcrm.2024.101415

Figure Lengend Snippet:

Article Snippet: Double-blinded quantitative detection of IFN-γ and CSF3 were performed using the mouse IFN-γ ELISA kit (R&D Systems, USA) and CSF3 ELISA kit (R&D Systems, USA) according to the manufacturer’s instructions.

Techniques: Virus, Recombinant, Red Blood Cell Lysis, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Isolation, Multiple Displacement Amplification, Expressing, shRNA, Software

FIGURE 6 Levels of IL-6, CSF3, MMP8, and S100A8 and ROC curves of S100A8 and MMP8 in the peripheral blood of SAE patients. (A) When compared to the non-SAE (n=10) group, the SAE patients (n=21) had higher serum levels of MMP8 and S100A8 (both p<0.0). (B) High diagnostic accuracy was shown for the identiﬁcation of SAE by both S100A8 (AUC = 0.962, 95% CI = 0.9001-1.000) and MMP8 (AUC = 0.7905, 95% CI = 0.6245-0.9564), with S100A8 having the highest accuracy. (C, D) Correlation analysis of the four extracellular molecules with clinical indicators and prognostic markers. S100A8, MMP8, CSF3, and IL-6 protein levels in the peripheral blood of sepsis patients were signiﬁcantly linked with GCS scores (all p<0.05). S100A8 levels and 28-day mortality were strongly associated (r=0.634, p<0.01), and IL-6 levels and duration of mechanical breathing were positively correlated (r=0.360, p=0.046). *p <0.05, **p <0.01, ****p <0.0001; ns, no signiﬁcance.

Journal: Frontiers in immunology

Article Title: Extracellular proteins as potential biomarkers in Sepsis-related cerebral injury.

doi: 10.3389/fimmu.2023.1128476

Figure Lengend Snippet: FIGURE 6 Levels of IL-6, CSF3, MMP8, and S100A8 and ROC curves of S100A8 and MMP8 in the peripheral blood of SAE patients. (A) When compared to the non-SAE (n=10) group, the SAE patients (n=21) had higher serum levels of MMP8 and S100A8 (both p<0.0). (B) High diagnostic accuracy was shown for the identiﬁcation of SAE by both S100A8 (AUC = 0.962, 95% CI = 0.9001-1.000) and MMP8 (AUC = 0.7905, 95% CI = 0.6245-0.9564), with S100A8 having the highest accuracy. (C, D) Correlation analysis of the four extracellular molecules with clinical indicators and prognostic markers. S100A8, MMP8, CSF3, and IL-6 protein levels in the peripheral blood of sepsis patients were signiﬁcantly linked with GCS scores (all p<0.05). S100A8 levels and 28-day mortality were strongly associated (r=0.634, p<0.01), and IL-6 levels and duration of mechanical breathing were positively correlated (r=0.360, p=0.046). *p <0.05, **p <0.01, ****p <0.0001; ns, no signiﬁcance.

Article Snippet: The levels of IL6, MMP8, CSF3, and S100A8 were determined by the following commercial ELISA kits, according to manufacturer’s recommendations: MMP8 (Cat.#CSB-E04680h, Cusabio Biotech Co. Ltd, Wuhan, China), S100A8 (Cat. #CSB-E11833h, Cusabio Biotech Co. Ltd, Wuhan, China), IL-6 (Cat. #CSB-E04638h, Cusabio Biotech Co. Ltd, Wuhan, China), and CSF3 (Cat. #EK0360, Boster Biotech Co. Ltd, USA).

Techniques: Diagnostic Assay

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